%0 Journal Article %J Nature Methods %D 2021 %T Chronically implantable LED arrays for behavioral optogenetics in primates %A Rajalingham, Rishi %A Sorenson, Michael %A Azadi, Reza %A Bohn, Simon %A DiCarlo, James J. %A Afraz, Arash %X

Optogenetic methods have been widely used in rodent brains, but remain relatively under-developed for nonhuman primates such as rhesus macaques, an animal model with a large brain expressing sophisticated sensory, motor and cognitive behaviors. To address challenges in behavioral optogenetics in large brains, we developed Opto-Array, a chronically implantable array of light-emitting diodes for high-throughput optogenetic perturbation. We demonstrated that optogenetic silencing in the macaque primary visual cortex with the help of the Opto-Array results in reliable retinotopic visual deficits in a luminance discrimination task. We separately confirmed that Opto-Array illumination results in local neural silencing, and that behavioral effects are not due to tissue heating. These results demonstrate the effectiveness of the Opto-Array for behavioral optogenetic applications in large brains.

%B Nature Methods %V 18 %P 1112 - 1116 %8 Jan-09-2021 %G eng %U https://www.nature.com/articles/s41592-021-01238-9 %N 9 %! Nat Methods %R 10.1038/s41592-021-01238-9 %0 Journal Article %J bioRxiv %D 2020 %T Chronically implantable LED arrays for behavioral optogenetics in primates %A Rajalingham, Rish %A Sorenson, Michael %A Azadi, Reza %A Bohn, Simon %A DiCarlo, James J. %A Afraz, Arash %X

Challenges in behavioral optogenetics in large brains demand development of a chronically implantable platform for light delivery. We have developed Opto-Array, a chronically implantable array of LEDs for high-throughput optogenetic perturbation in non-human primates. We tested the Opto-Array in the primary visual cortex of a macaque monkey, and demonstrated that optogenetic cortical silencing by the Opto-Array results in reliable retinotopic visual deficits on a luminance discrimination task.

%B bioRxiv %8 9/11/2020 %G eng %U https://www.biorxiv.org/content/10.1101/2020.09.10.291583v1.abstract %9 preprint %R 10.1101/2020.09.10.291583