Title | Chronically implantable LED arrays for behavioral optogenetics in primates |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Rajalingham, R, Sorenson, M, Azadi, R, Bohn, S, DiCarlo, JJ, Afraz, A |
Journal | Nature Methods |
Volume | 18 |
Issue | 9 |
Pagination | 1112 - 1116 |
Date Published | Jan-09-2021 |
ISSN | 1548-7091 |
Abstract | Optogenetic methods have been widely used in rodent brains, but remain relatively under-developed for nonhuman primates such as rhesus macaques, an animal model with a large brain expressing sophisticated sensory, motor and cognitive behaviors. To address challenges in behavioral optogenetics in large brains, we developed Opto-Array, a chronically implantable array of light-emitting diodes for high-throughput optogenetic perturbation. We demonstrated that optogenetic silencing in the macaque primary visual cortex with the help of the Opto-Array results in reliable retinotopic visual deficits in a luminance discrimination task. We separately confirmed that Opto-Array illumination results in local neural silencing, and that behavioral effects are not due to tissue heating. These results demonstrate the effectiveness of the Opto-Array for behavioral optogenetic applications in large brains. |
URL | https://www.nature.com/articles/s41592-021-01238-9 |
DOI | 10.1038/s41592-021-01238-9 |
Short Title | Nat Methods |